Emory University School of Medicine
Emory University School of Medicine
Department of Pharmacology and Chemical Biology

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Department of Pharmacology and Chemical Biology

Publications

The Kukar Lab is part of the Department of Pharmacology and Chemical Biology

Papers

  1. Regulation of TFEB nuclear localization by HSP90AA1 promotes autophagy and longevity.
    Autophagy Page(s): 1 - 17
    08/08/2022 Authors: Yang S; Nie T; She H; Tao K; Lu F; Hu Y; Huang L; Zhu L; Feng D; He D
  2. Proximity-based labeling reveals DNA damage-induced phosphorylation of fused in sarcoma (FUS) causes distinct changes in the FUS protein interactome.
    J Biol Chem Page(s): 102135
    06/13/2022 Authors: Johnson MA; Nuckols TA; Merino P; Bagchi P; Nandy S; Root J; Taylor G; Seyfried NT; Kukar T
  3. Lysosomal Dysfunction and Other Pathomechanisms in FTLD: Evidence from Progranulin Genetics and Biology. Zhou X., Kukar T., Rademakers R. Adv Exp Med Biol. 2021;1281:219-242. doi: 10.1007/978-3-030-51140-1_14. PMID: 33433878; PMCID: PMC8672701.
  4. Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations. Huang M., Modeste E, Dammer, E.B., Merino, P, Taylor, G., Duong, D.M., Deng, Q., Holler, C. J., Gearing, M., Dickson, D., Seyfried N.T., and Kukar, T. 
    Acta Neuropatholgica Communications, 7; 2020, 8(1):163.
    PMID: 33028409
  5. Divergent FUS phosphorylation in primate and mouse cells following double-strand DNA damage. Johnson M.A., Deng, Q., Taylor, G., McEachin, Z.T., Chan W.S.A., Root, J., Bassell, G.J., Kukar, T.
    Neurobiology of Disease,     2020, 18;146:105085
    PMID: 32950644
  6. Chimeric Peptide Species Contribute to Divergent Dipeptide Repeat Pathology in c9ALS/FTD and SCA36. Z.T. McEachin, T.F. Gendron, N. Raj, M. García-Murias, A. Banerjee, P.J. Ward, T. Todd, M.E. Merritt-Garza, K. Jansen-West, C.M. Hales, T. García-Sobrino, B. Quintáns, C. Holler, G. Taylor, B. San Millan, S. Teijeira, T. Yamashita, R. Ohkubo, N. Boulis, C. Xu, Z. Wen, N. Streichenberger, Neuro-CEB Neuropathology Network, B.L. Fogel, T. Kukar, K. Abe, D. Dickson, M. Arias, J.D. Glass, J. Jiang, M. Tansey, M-J Sobrido, L. Petrucelli, W. Rossoll, G.J. Bassell. 
    Neuron, 2020 Jul 22;107(2):292-305.
    PMID: 32375063
  7. Molecular Signatures of Neuroinflammation Induced by α-Synuclein Aggregates in Microglial Cells. Sarkar S., Dammer E.B., Malovic E., Olsen A.L., Raza S.A., Gao T., Xiao H., Oliver D.L., Duong D., Joers V., Seyfried N., Huang M., Kukar T., Tansey M.G., Kanthasamy A.G., Rangaraju S.
    Front Immunol, 2020, 11:33.
    PMID: 32082315
  8. TDP-43 pathology disrupts nuclear pore complexes and nucleocytoplasmic transport in ALS/FTD. Chou C.C. , Zhang Y., Umoh M., Vaughan S., Lorenzini L., Liu F., Sayegh M., Donlin-Asp P., Chen Y., Duong D., Seyfried N., Powers M., Kukar T., Hales C., Gearing M., Cairns N., Boylan K., Dickson D., Rademakers R., Zhang Y.J., Petrucelli L., Sattler R., Zarnescu D.,  Glass J., Rossoll W. 
    Nature Neuroscience, 2018 Feb;21(2):228-239.
    PMID: 2931174
  9. Short Aβ peptides attenuate Aβ42 toxicity in vivo. Moore BD, Martin J, de Mena L, Sanchez J, Cruz PE1 Ceballos-Diaz C, Ladd TB, Ran Y, Levites Y, Kukar TL, Kurian JJ, McKenna R, Koo EH, Borchelt DR, Janus C, Rincon-Limas , D Fernandez-Funez P, Golde TE
     2018 Jan 2;215(1):283-301. doi: 10.1084/jem.20170600. Epub 2017 Dec 5.
    PMID: 29208777 
  10. Intracellular proteolysis of progranulin generates stable, lysosomal granulins that are haploinsufficient in patients with frontotemporal dementia caused by GRN mutations.Holler CJ, Taylor G, Deng Q, and Kukar T.
    eNeuro 9 August 2017, 4 (4) ENEURO.0100-17.2017; DOI: https://doi.org/10.1523/ENEURO.0100-17.2017
    PMID: 28828399.
  11. Disulfide bonds and disorder in granulin-3: An unusual handshake between structural stability and plasticity.Ghag G, Holler CJ, Taylor G, Kukar TL, Uversky VN, Rangachari V.
    Protein Sci. 2017 Jun 12. doi: 10.1002/pro.3212.
    PMID: 28608407
  12. Trehalose upregulates progranulin expression in human and mouse models of GRN haploinsufficiency: a novel therapeutic lead to treat frontotemporal dementia.Holler CJ, Taylor G, McEachin ZT, Deng Q, Watkins WJ, Hudson K, Easley CA, Hu WT, Hales CM, Rossoll W, Bassell GJ, Kukar T
    Mol Neurodegener. 2016 Jun 24;11(1):46. doi: 10.1186/s13024-016-0114-3
    PMID: 27341800
  13. Complex relationships between substrate sequence and sensitivity to alterations in gamma-secretase processivity induced by gamma-secretase modulators.
    Jung JI, Ran Y, Cruz PE, Rosario AM, Ladd TB, Kukar TL, Koo EH, Felsenstein KM, Golde TE.
    Biochemistry. 2014 Apr 1;53(12):1947-57. doi: 10.1021/bi401521t. Epub 2014 Mar 20.
    PMID: 24620716
  14. FUS is phosphorylated by DNA-PK and accumulates in the cytoplasm after DNA damage.Deng Q, Holler CJ, Taylor G, Hudson KF, Watkins W, Gearing M, Ito D, Murray ME, Dickson DW, Seyfried NT, Kukar T.
    J Neurosci. 2014 Jun 4;34(23):7802-13. doi: 10.1523/JNEUROSCI.0172-14.2014.
    PMID: 24899704
  15. Steroids as gamma-secretase modulators.Jung JI, Ladd TB, Kukar T, Price AR, Moore BD, Koo EH, Golde TE, Felsenstein KM.
    FASEB J. 2013 Sep;27(9):3775-85. doi: 10.1096/fj.12-225649. Epub 2013 May 28.
    PMID: 23716494
  16. Progranulin does not bind tumor necrosis factor (TNF) receptors and is not a direct regulator of TNF-dependent signaling or bioactivity in immune or neuronal cells.Chen X1, Chang J, Deng Q, Xu J, Nguyen TA, Martens LH, Cenik B, Taylor G, Hudson KF, Chung J, Yu K, Yu P, Herz J, Farese RV Jr, Kukar T, Tansey MG.
    J Neurosci. 2013 May 22;33(21):9202-13. doi: 10.1523/JNEUROSCI.5336-12.2013.
    PMID: 23699531
  17. Expression of Fused in sarcoma mutations in mice recapitulates the neuropathology of FUS proteinopathies and provides insight into disease pathogenesis.Verbeeck C, Deng Q, Dejesus-Hernandez M, Taylor G, Ceballos-Diaz C, Kocerha J, Golde T, Das P, Rademakers R, Dickson DW, Kukar T.
    Mol Neurodegener. 2012 Oct 10;7:53. doi: 10.1186/1750-1326-7-53.
    PMID: 23046583
  18. Overlapping profiles of ABeta peptides in the Alzheimer's disease and pathological aging brains.Moore BD, Chakrabarty P, Levites Y, Kukar TL, Baine AM, Moroni T, Ladd TB, Das P, Dickson DW, Golde TE.
    Alzheimers Res Ther. 2012 May 23;4(3):18. doi: 10.1186/alzrt121
    PMID: 22621179
  19. Transient pharmacologic lowering of Abeta production prior to deposition results in sustained reduction of amyloid plaque pathologyDas P, Verbeeck C, Minter L, Chakrabarty P, Felsenstein K, Kukar T, Maharvi G, Fauq A, Osborne BA, Gold TE. 
    Mol Neurodegener. 2012 Aug 14;7:39. doi: 10.1186/1750-1326-7-39
    PMID: 22892055
  20. Substrate sequence influences gamma-secretase modulator activity, role of the transmembrane domain of the amyloid precursor proteinSagi SA, Lessard CB, Winden KD, Maruyama H, Koo JC, Weggen S, Kukar TL, Golde TE, Koo EH.
    J Biol Chem. 2011 Nov 18;286(46):39794-803. doi: 10.1074/jbc.M111.277228. Epub 2011 Aug 25
    PMID: 21868380
  21. Lysine 624 of the amyloid precursor protein (APP) is a critical determinant of amyloid beta peptide length: support for a sequential model of gamma-secretase intramembrane proteolysis and regulation by the amyloid beta precursor protein (APP) juxtamembrane region.Kukar TL, Ladd TB, Robertson P, Pintchovski SA, Moore B, Bann MA, Ren Z, Jansen-West K, Malphrus K, Eggert S, Maruyama H, Cottrell BA, Das P, Basi GS, Koo EH, Golde TE.
    J Biol Chem. 2011 Nov 18;286(46):39804-12. doi: 10.1074/jbc.M111.274696. Epub 2011 Aug 25
    PMID: 21868378
  22. An NSAID-like compound, FT-9, preferentially inhibits gamma-secretase cleavage of the amyloid precursor protein compared to its effect on amyloid precursor-like protein 1.Sala Frigerio C, Kukar TL, Fauq A, Engel PC, Golde TE, Walsh DM.
    Biochemistry. 2009 Nov 24;48(46):10894-904.
    PMID: 19821615
  23. Substrate-targeting gamma-secretase modulators.Kukar TL, Ladd TB, Bann MA, Fraering PC, Narlawar R, Maharvi GM, Healy B, Chapman R, Welzel AT, Price RW, Moore B, Rangachari V, Cusack B, Eriksen J, Jansen-West K, Verbeeck C, Yager D, Eckman C, Ye W, Sagi S, Cottrell BA, Torpey J, Rosenberry TL, Fauq A, Wolfe MS, Schmidt B, Walsh DM, Koo EH, Golde TE.
    Nature. 2008 Jun 12;453(7197):925-9.
    PMID: 18548070
  24. Independent generation of Abeta42 and Abeta38 peptide species by gamma-secretase.Czirr E, Cottrell BA, Leuchtenberger S, Kukar T, Ladd TB, Esselmann H, Paul S, Schubenel R, Torpey JW, Pietrzik CU, Golde TE, Wiltfang J, Baumann K, Koo EH, Weggen S.
    J Biol Chem. 2008 Jun 20;283(25):17049-54. Epub 2008 Apr 21.
    PMID: 18426795
  25. Chronic administration of R-flurbiprofen attenuates learning impairments in transgenic amyloid precursor protein mice.Kukar T, Prescott S, Eriksen JL, Holloway V, Murphy MP, Koo EH, Golde TE, Nicolle MM.
    BMC Neurosci. 2007 Jul 24;8:54.
    PMID: 17650315
  26. Intramembrane proteolytic cleavage by human signal peptide peptidase like 3 and malaria signal peptide peptidase.Nyborg AC, Ladd TB, Jansen K, Kukar T, Golde TE.
    FASEB J. 2006 Aug;20(10):1671-9.
    PMID: 16873890 [PubMed - indexed for MEDLINE] Free Article
  27. Inhibitors of Rho-kinase modulate amyloid-beta (Abeta) secretion but lack selectivity for Abeta42.Leuchtenberger S, Kummer MP, Kukar T, Czirr E, Teusch N, Sagi SA, Berdeaux R, Pietrzik CU, Ladd TB, Golde TE, Koo EH, Weggen S.
    J Neurochem. 2006 Jan;96(2):355-65. Epub 2005 Nov 21.
    PMID: 16300630
  28. Diverse compounds mimic Alzheimer disease-causing mutations by augmenting Abeta42 production.Kukar T, Murphy MP, Eriksen JL, Sagi SA, Weggen S, Smith TE, Ladd T, Khan MA, Kache R, Beard J, Dodson M, Merit S, Ozols VV, Anastasiadis PZ, Das P, Fauq A, Koo EH, Golde TE.
    Nat Med. 2005 May;11(5):545-50. Epub 2005 Apr 17.
    PMID: 15834426
  29. Structural insights and functional implications of choline acetyltransferase.
    Govindasamy L, Pedersen B, Lian W, Kukar T, Gu Y, Jin S, Agbandje-McKenna M, Wu D, McKenna R.J 
    Struct Biol. 2004 Nov;148(2):226-35.
    PMID: 15477102
  30. Structural and mutational characterization of L-carnitine binding to human carnitine acetyltransferase.Govindasamy L, Kukar T, Lian W, Pedersen B, Gu Y, Agbandje-McKenna M, Jin S, McKenna R, Wu D.
    J Struct Biol. 2004 Jun;146(3):416-24.
    PMID: 15099582
  31. Crystallization and preliminary X-ray crystallographic studies on recombinant rat choline acetyltransferase.Lian W, Gu Y, Pedersen B, Kukar T, Govindasamy L, Agbandje-McKenna M, Jin S, McKenna R, Wu D.
    Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):374-5. Epub 2004 Jan 23.
    PMID: 14747730
  32. Structure of human carnitine acetyltransferase. Molecular basis for fatty acyl transfer.Wu D, Govindasamy L, Lian W, Gu Y, Kukar T, Agbandje-McKenna M, McKenna R.
    J Biol Chem. 2003 Apr 11;278(15):13159-65. Epub 2003 Jan 31.
    PMID: 12562770
  33. Protein microarrays to detect protein-protein interactions using red and green fluorescent proteins.Kukar T, Eckenrode S, Gu Y, Lian W, Megginson M, She JX, Wu D.
    Anal Biochem. 2002 Jul 1;306(1):50-4.
    PMID: 12069413
  34. Crystallization and preliminary X-ray crystallographic studies on recombinant human carnitine acetyltransferase.Lian W, Govindasamy L, Gu Y, Kukar T, Agbandje-McKenna M, McKenna R, Wu D.
    Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1193-4. Epub 2002 Jun 20.
    PMID: 12077440
  35. Molecular cloning and characterization of the mouse and human TUSP gene, a novel member of the tubby superfamily.Li QZ, Wang CY, Shi JD, Ruan QG, Eckenrode S, Davoodi-Semiromi A, Kukar T, Gu Y, Lian W, Wu D, She JX.
    Gene. 2001 Aug 8;273(2):275-84.
    PMID: 11595174
  36. Molecular cloning and characterization of a novel mammalian endo-apyrase (LALP1).Shi JD, Kukar T, Wang CY, Li QZ, Cruz PE, Davoodi-Semiromi A, Yang P, Gu Y, Lian W, Wu DH, She JX.
    J Biol Chem. 2001 May 18;276(20):17474-8. Epub 2001 Feb 23.
    PMID: 11278936


Reviews

  1. Defects in RNA Metabolism links FTD and ALS Pathogenesis: TDP-43, FUS, and C9orf72.
     Deng Q, Chalermpalanupap T, Kukar, T.
    Current Enzyme Inhibition 9, 28-40.
  2. Medicine. Avoiding unintended toxicity.
     Golde TE, Kukar TL.
    Science. 2009 May 1;324(5927):603-4. doi: 10.1126/science.1174267.
    PMID: 19407192
  3. Possible mechanisms of action of NSAIDs and related compounds that modulate gamma-secretase cleavage.
    Kukar T, Golde TE.
    Curr Top Med Chem. 2008;8(1):47-53
    PMID: 18220932