Ling Ye PhD
- Department of Microbiology and Immunology
Assistant Professor
- (404) 727-6558
- yling@emory.edu
-
Emory University School of Medicine
Microbiology and Immunology
1510 Clifton Rd., Room 3086 RRC
Overview
The main focus of my research interest is to design novel vaccine strategies for eliciting broadly neutralizing antibodies against HIV. In particular, we have constructed a chimeric protein, HA/gp41, by fusing the HIV Env gp41 in frame to the C-terminus of the HA1 subunit of an influenza virus hemagglutinin. The HA/gp41 is efficiently transported to the cell surfaces, exhibits enhanced reactivity to monoclonal antibodies 2F5 and 4E10, and more effectively induces gp41-specific antibodies compared to the HIV Env. Recently, we further evaluated the immunogenicity of HA/gp41-based DNA or virus-like particle (VLP) vaccines in guinea pigs and found that immunization with HA/gp41 DNA or VLP vaccines induced neutralizing antibodies against the conserved epitopes in gp41. Currently, we are evaluating the effectiveness of this chimeric protein vaccine in small animal models, with the goal to improve its design for optimizing its ability to induce broadly HIV neutralizing antibodies. In addition to HIV vaccine development, we are also investigating the role of regulatory T cells in modulating vaccine-induced immune responses in aging host using the mouse model.
Academic Appointment
- Assistant Professor, Department of Microbiology and Immunology, Emory University School of Medicine
Education
Degrees
- MD, PhD from Fourth Military Medical University
- M.S. from Shanxi Medical University
- BS from Shanxi Medical University
Research
Focus
- My research interests center on developing virus-like particle vaccines against virus infection. Currently, we are investigating the potential of incorporating immune stimulating molecules to enhance immunogenicities of virus-like particles and modification of viral glycoproteins to improve their antigenicities for obtaining more effective vaccines against HIV, Ebola virus, influenza virus, and hepatitis C virus.
Publications
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An ultra-low-cost electroporator with microneedle electrodes (ePatch) for SARS-CoV-2 vaccination.
Proc Natl Acad Sci U S A Volume: 118
11/09/2021 Authors: Xia D; Jin R; Byagathvalli G; Yu H; Ye L; Lu C-Y; Bhamla MS; Yang C; Prausnitz MR -
Intradermal Immunization of EBOV VLPs in Guinea Pigs Induces Broader Antibody Responses Against GP Than Intramuscular Injection.
Front Microbiol Volume: 11 Page(s): 304
01/01/2020 Authors: Liu Y; Wen Z; Carrion R; Nunneley J; Staples H; Ticer A; Patterson JL; Compans RW; Ye L; Yang C -
Transplantation of fecal microbiota rich in short chain fatty acids and butyric acid treat cerebral ischemic stroke by regulating gut microbiota.
Pharmacol Res Volume: 148 Page(s): 104403
10/01/2019 Authors: Chen R; Xu Y; Wu P; Zhou H; Lasanajak Y; Fang Y; Tang L; Ye L; Li X; Cai Z -
Intradermal Vaccination With Adjuvanted Ebola Virus Soluble Glycoprotein Subunit Vaccine by Microneedle Patches Protects Mice Against Lethal Ebola Virus Challenge.
J Infect Dis Volume: 218 Page(s): S545 - S552
11/22/2018 Authors: Liu Y; Ye L; Lin F; Gomaa Y; Flyer D; Carrion R; Patterson JL; Prausnitz MR; Smith G; Glenn G -
Intradermal immunization by Ebola virus GP subunit vaccines using microneedle patches protects mice against lethal EBOV challenge.
Sci Rep Volume: 8 Page(s): 11193
07/25/2018 Authors: Liu Y; Ye L; Lin F; Gomaa Y; Flyer D; Carrion R; Patterson JL; Prausnitz MR; Smith G; Glenn G -
Blockage of regulatory T cells augments induction of protective immune responses by influenza virus-like particles in aged mice.
Microbes Infect Volume: 19 Page(s): 626 - 634
12/01/2017 Authors: Wen Z; Wang X; Dong K; Zhang H; Bu Z; Ye L; Yang C -
Site-specific Isopeptide Bridge Tethering of Chimeric gp41 N-terminal Heptad Repeat Helical Trimers for the Treatment of HIV-1 Infection.
Sci Rep Volume: 6 Page(s): 32161
08/26/2016 Authors: Wang C; Li X; Yu F; Lu L; Jiang X; Xu X; Wang H; Lai W; Zhang T; Zhang Z -
An immunogen containing four tandem 10E8 epitope repeats with exposed key residues induces antibodies that neutralize HIV-1 and activates an ADCC reporter gene.
Emerg Microbes Infect Volume: 5 Page(s): e65
06/22/2016 Authors: Sun Z; Zhu Y; Wang Q; Ye L; Dai Y; Su S; Yu F; Ying T; Yang C; Jiang S -
Production of Potent Fully Human Polyclonal Antibodies against Ebola Zaire Virus in Transchromosomal Cattle.
Sci Rep Volume: 6 Page(s): 24897
04/25/2016 Authors: Dye JM; Wu H; Hooper JW; Khurana S; Kuehne AI; Coyle EM; Ortiz RA; Fuentes S; Herbert AS; Golding H -
Enhanced Stability of Inactivated Influenza Vaccine Encapsulated in Dissolving Microneedle Patches.
Pharm Res Volume: 33 Page(s): 868 - 878
04/01/2016 Authors: Chu LY; Ye L; Dong K; Compans RW; Yang C; Prausnitz MR