Publications

• iCLOTS: open-source, artificial intelligence-enabled software for analyses of blood cells in microfluidic and microscopy-based assays.
  Nat Commun Volume: 14 Page(s): 5022
  08/18/2023 Authors: Fay ME; Oshinowo O; Iffrig E; Fibben KS; Caruso C; Hansen S; Musick JO; Valdez JM; Azer SS; Mannino RG
• The critical role of engineering in the rapid development of COVID-19 diagnostics: Lessons from the RADx Tech Test Verification Core.
  Sci Adv Volume: 9 Page(s): eade4962
  04/07/2023 Authors: Mannino RG; Nehl EJ; Farmer S; Peagler AF; Parsell MC; Claveria V; Ku D; Gottfried DS; Chen H; Lam WA
• Navigating the complexities of mobile medical app development from idea to launch, a guide for clinicians and biomedical researchers.
  BMC Med Volume: 21 Page(s): 109
  03/23/2023 Authors: Mannino RG; Arconada Alvarez SJ; Greenleaf M; Parsell M; Mwalija C; Lam WA
• Don't forget about human factors: Lessons learned from COVID-19 point-of-care testing.
  Cell Rep Methods Volume: 2 Page(s): 100222
  05/23/2022 Authors: Farmer S; Razin V; Peagler AF; Strickler S; Fain WB; Damhorst GL; Kempker RR; Pollock NR; Brand O; Seitter B
• The RADx Tech Test Verification Core and the ACME POCT in the Evaluation of COVID-19 Testing Devices: A Model for Progress and Change.
  IEEE Open J Eng Med Biol Volume: 2 Page(s): 142 - 151
  04/28/2021 Authors: Nehl E; Heilman S; Ku D; Gottfried D; Farmer S; Mannino R; Tyburski E; Sullivan J; Suessmith A; Bassit L
• Covid-19 will not "magically disappear": Why access to widespread testing is paramount.
  Am J Hematol Volume: 96 Page(s): 174 - 178
  02/01/2021 Authors: George PE; Stokes CL; Bassit LC; Chahroudi A; Figueroa J; Griffiths MA; Heilman S; Ku DN; Nehl EJ; Leong T
• A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits.
  J Biol Chem Volume: 295 Page(s): 15438 - 15453
  11/13/2020 Authors: Mascuch SJ; Fakhretaha-Aval S; Bowman JC; Ma MTH; Thomas G; Bommarius B; Ito C; Zhao L; Newnam GP; Matange KR
• A blueprint for academic labs to produce SARS-CoV-2 RT-qPCR test kits.
  medRxiv
  09/01/2020 Authors: Mascuch SJ; Fakhretaha-Aval S; Bowman JC; Ma MTH; Thomas G; Bommarius B; Ito C; Zhao L; Newnam GP; Matange KR
• Enabling mesenchymal stromal cell immunomodulatory analysis using scalable platforms.
  Integr Biol (Camb) Volume: 11 Page(s): 154 - 162
  04/01/2019 Authors: Williams EK; Garca JR; Mannino RG; Schneider RS; Lam WA; Garca AJ
• Integrated automated particle tracking microfluidic enables high-throughput cell deformability cytometry for red cell disorders.
  Am J Hematol Volume: 94 Page(s): 189 - 199
  02/01/2019 Authors: Guruprasad P; Mannino RG; Caruso C; Zhang H; Josephson CD; Roback JD; Lam WA
• Smartphone app for non-invasive detection of anemia using only patient-sourced photos.
  Nat Commun Volume: 9 Page(s): 4924
  12/04/2018 Authors: Mannino RG; Myers DR; Tyburski EA; Caruso C; Boudreaux J; Leong T; Clifford GD; Lam WA
• Integrated Microfluidic Automated Particle Tracking F:nables High-Throughput Cell Deformability Cytometry for Red Cell Disorders
  Volume: 132
  11/29/2018 Authors: Caruso C; Guruprasad P; Mannino RG; Zhang H; Josephson CD; Roback JD; Lam WA
• Interdigitated microelectronic bandage augments hemostasis and clot formation at low applied voltage in vitro and in vivo.
  Lab Chip Volume: 18 Page(s): 2985 - 2993
  09/26/2018 Authors: Hardy ET; Wang YJ; Iyer S; Mannino RG; Sakurai Y; Barker TH; Chi T; Youn Y; Wang H; Brown AC
• Protein Corona in Response to Flow: Effect on Protein Concentration and Structure.
  Biophys J Volume: 115 Page(s): 209 - 216
  07/17/2018 Authors: Jayaram DT; Pustulka SM; Mannino RG; Lam WA; Payne CK
• Endothelial cell culture in microfluidic devices for investigating microvascular processes.
  Biomicrofluidics Volume: 12 Page(s): 042203
  07/01/2018 Authors: Mannino RG; Qiu Y; Lam WA
• Thrombosis-on-a-chip: Prospective impact of microphysiological models of vascular thrombosis.
  Curr Opin Biomed Eng Volume: 5 Page(s): 29 - 34
  03/01/2018 Authors: Pandian NKR; Mannino RG; Lam WA; Jain A
• Engineering "Endothelialized" Microfluidics for Investigating Vascular and Hematologic Processes Using Non-Traditional Fabrication Techniques.
  Curr Opin Biomed Eng Volume: 5 Page(s): 13 - 20
  03/01/2018 Authors: Mannino RG; Pandian NK; Jain A; Lam WA
• A microengineered vascularized bleeding model that integrates the principal components of hemostasis.
  Nat Commun Volume: 9 Page(s): 509
  02/06/2018 Authors: Sakurai Y; Hardy ET; Ahn B; Tran R; Fay ME; Ciciliano JC; Mannino RG; Myers DR; Qiu Y; Carden MA
• 3D in vitro microvascular model-based lymphoma model.
  Volume: 146 Page(s): 149 - 158
  01/01/2018 Authors: Mannino RG; Pradhan P; Roy K; Lam WA
• Microvasculature-on-a-chip for the long-term study of endothelial barrier dysfunction and microvascular obstruction in disease.
  Nat Biomed Eng Volume: 2 Page(s): 453 - 463
  01/01/2018 Authors: Qiu Y; Ahn B; Sakurai Y; Hansen CE; Tran R; Mimche PN; Mannino RG; Ciciliano JC; Lamb TJ; Joiner CH
• Extracellular fluid tonicity impacts sickle red blood cell deformability and adhesion.
  Blood Volume: 130 Page(s): 2654 - 2663
  12/14/2017 Authors: Carden MA; Fay ME; Lu X; Mannino RG; Sakurai Y; Ciciliano JC; Hansen CE; Chonat S; Joiner CH; Wood DK
• Single-platelet nanomechanics measured by high-throughput cytometry.
  Nat Mater Volume: 16 Page(s): 230 - 235
  02/01/2017 Authors: Myers DR; Qiu Y; Fay ME; Tennenbaum M; Chester D; Cuadrado J; Sakurai Y; Baek J; Tran R; Ciciliano JC
• 3D microvascular model recapitulates the diffuse large B-cell lymphoma tumor microenvironment in vitro.
  Lab Chip Volume: 17 Page(s): 407 - 414
  01/31/2017 Authors: Mannino RG; Santiago-Miranda AN; Pradhan P; Qiu Y; Mejias JC; Neelapu SS; Roy K; Lam WA
• Cellular softening mediates leukocyte demargination and trafficking, thereby increasing clinical blood counts.
  Proc Natl Acad Sci U S A Volume: 113 Page(s): 1987 - 1992
  02/23/2016 Authors: Fay ME; Myers DR; Kumar A; Turbyfield CT; Byler R; Crawford K; Mannino RG; Laohapant A; Tyburski EA; Sakurai Y
• Vessel Geometry Interacts with Red Blood Cell Stiffness to Promote Endothelial Dysfunction in Sickle Cell Disease
  Volume: 126
  12/03/2015 Authors: Wang Y; Mannino RG; Myers DR; Li W; Joiner CH; Lam WA
• "Do-it-yourself in vitro vasculature that recapitulates in vivo geometries for investigating endothelial-blood cell interactions".
  Sci Rep Volume: 5 Page(s): 12401
  07/23/2015 Authors: Mannino RG; Myers DR; Ahn B; Wang Y; Margo Rollins; Gole H; Lin AS; Guldberg RE; Giddens DP; Timmins LH
• Platelet Adhesion, Spreading and Activation Are Mediated By Mechanosensing of Matrix Stiffness
  Volume: 124
  12/06/2014 Authors: Qiu Y; Brown AC; Myers DR; Sakurai Y; Mannino R; Tran R; Ahn B; Hardy E; Kee MF; Kumar S
• Platelet mechanosensing of substrate stiffness during clot formation mediates adhesion, spreading, and activation.
  Proc Natl Acad Sci U S A Volume: 111 Page(s): 14430 - 14435
  10/07/2014 Authors: Qiu Y; Brown AC; Myers DR; Sakurai Y; Mannino RG; Tran R; Ahn B; Hardy ET; Kee MF; Kumar S
• Disposable platform provides visual and color-based point-of-care anemia self-testing.
  J Clin Invest Volume: 124 Page(s): 4387 - 4394
  10/01/2014 Authors: Tyburski EA; Gillespie SE; Stoy WA; Mannino RG; Weiss AJ; Siu AF; Bulloch RH; Thota K; Cardenas A; Session W
• Biomechanics of haemostasis and thrombosis in health and disease: from the macro- to molecular scale.
  J Cell Mol Med Volume: 17 Page(s): 579 - 596
  05/01/2013 Authors: Tran R; Myers DR; Ciciliano J; Trybus Hardy EL; Sakurai Y; Ahn B; Qiu Y; Mannino RG; Fay ME; Lam WA
• An "Endothelialized" Microfluidic System That Distinguishes Procoagulant Mechanisms in Arterial and Venous Thrombosis
  Volume: 120
  11/16/2012 Authors: Ahn B; Sakurai Y; Myers DR; Qiu Y; Hardy E; Tran R; Mannino R; Wolberg AS; Lam W
• Endothelialized microfluidics for studying microvascular interactions in hematologic diseases.
  J Vis Exp
  06/22/2012 Authors: Myers DR; Sakurai Y; Tran R; Ahn B; Hardy ET; Mannino R; Kita A; Tsai M; Lam WA