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Profile

James Zheng PhD

  • Department of Cell Biology
    Professor
  • (404) 727-9133
  • jqzheng@emory.edu
  • Zheng Lab Website
Head shot of James Zheng

Overview

We study the cell biology of nerve cells with a specific focus on the signal transduction and cytoskeletal mechanisms underlying neural development, disorders, and regeneration. Three major areas are being pursued in our lab: 1- axonal growth and guidance; 2- synapse formation and plasticity; and 3- intracellular trafficking. Our research employs some unique experimental approaches that combine sophisticated molecular/cellular imaging and powerful molecular manipulation to perform functional analyses of neurons at single molecule level. Our goal is to not only gain a mechanistic understanding of the molecular and cellular aspects of neuronal structure and function, but also provide insights into the cellular basis for brain abnormality and disorders. It is our hope that these basic studies will build the foundation for the development of potential strategies and treatments to promote regeneration and repair of damaged neuronal circuitry after neural injuries and degeneration.

Academic Appointment

  • Professor, Department of Neurology, Emory University School of Medicine
  • Professor with tenure, Department of Cell Biology, Emory University School of Medicine

Education

Degrees

  • PhD from Tsinghua University
  • BS from Tsinghua University

Research

Focus

  • Precisely-wired neuronal circuitry underlies the proper and complex functions of the nervous system. We investigate the molecular and cellular mechanisms underlying a variety of developmental events that lead to the construction of the complex nervous system. Our current focus is on the signal transduction and cytoskeletal mechanisms underlying neuronal migration, axon growth and guidance, and synaptic plasticity.

Publications

  • A Critical Role for the Fascin Family of Actin Bundling Proteins in Axon Development, Brain Wiring and Function.
    bioRxiv
    05/07/2025 Authors: Hardin KR; Penas AB; Joubert S; Ye C; Myers KR; Zheng JQ
  • Adverse impact of female reproductive signaling on age-dependent neurodegeneration after mild head trauma in Drosophila.

    08/30/2024 Authors: Ye C; Ho R; Moberg KH; Zheng JQ
  • Sexual Dimorphism in Age-Dependent Neurodegeneration After Mild Head Trauma in Drosophila : Unveiling the Adverse Impact of Female Reproductive Signaling.

    06/25/2024 Authors: Ye C; Ho R; Moberg KH; Zheng JQ
  • An enhancer-AAV approach selectively targeting dentate granule cells of the mouse hippocampus.
    Cell Rep Methods Volume: 4 Page(s): 100684
    01/22/2024 Authors: Banks E; Gutekunst C-A; Vargish GA; Eaton A; Pelkey KA; McBain CJ; Zheng JQ; Olh VJ; Rowan MJM
  • A novel enhancer-AAV approach selectively targeting dentate granule cells.
    bioRxiv
    05/11/2023 Authors: Banks E; Gutekunst C-A; Vargish GA; Eaton A; Pelkey KA; McBain CJ; Zheng JQ; Olh VJ; Rowan MJ
  • Drosophila melanogaster as a model to study age and sex differences in brain injury and neurodegeneration after mild head trauma.
    Front Neurosci Volume: 17 Page(s): 1150694
    01/01/2023 Authors: Ye C; Behnke JA; Hardin KR; Zheng JQ
  • Characterizing spontaneous Ca2+ local transients inOPCs using computational modeling.
    Biophys J Volume: 121 Page(s): 4419 - 4432
    12/06/2022 Authors: Oprea L; Desjardins N; Jiang X; Sareen K; Zheng JQ; Khadra A
  • Signal amplification in growth cone gradient sensing by a double negative feedback loop among PTEN, PI(3,4,5)P3 and actomyosin.
    Mol Cell Neurosci Volume: 123 Page(s): 103772
    12/01/2022 Authors: Li X; Shim S; Hardin KR; Vanaja KG; Song H; Levchenko A; Ming G-L; Zheng JQ
  • Virally Mediated Connexin 26 Expression in Postnatal Scala Media Significantly and Transiently Preserves Hearing in Connexin 30 Null Mice.
    Front Cell Dev Biol Volume: 10 Page(s): 900416
    01/01/2022 Authors: Zhang L; Wang W; Kim SM; Wang J; Zhou B; Kong W; Zheng J; Lin X
  • Corrigendum: Virally mediated connexin 26 expression in postnatal scala media significantly and transiently preserves hearing in connexin 30 null mice.
    Front Cell Dev Biol Volume: 10 Page(s): 969989
    01/01/2022 Authors: Zhang L; Wang W; Kim SM; Wang J; Zhou B; Kong W; Zheng J; Lin X
  • Actin capping protein regulates postsynaptic spine development through CPI-motif interactions.
    Front Mol Neurosci Volume: 15 Page(s): 1020949
    01/01/2022 Authors: Myers KR; Fan Y; McConnell P; Cooper JA; Zheng JQ
  • A protocol to detect neurodegeneration in Drosophila melanogaster whole-brain mounts using advanced microscopy.
    STAR Protoc Volume: 2 Page(s): 100689
    09/17/2021 Authors: Behnke JA; Ye C; Moberg KH; Zheng JQ
  • Repetitive mild head trauma induces activity mediated lifelong brain deficits in a novel Drosophila model.
    Sci Rep Volume: 11 Page(s): 9738
    05/06/2021 Authors: Behnke JA; Ye C; Setty A; Moberg KH; Zheng JQ
  • LIM and SH3 protein 1 localizes to the leading edge of protruding lamellipodia and regulates axon development.
    Mol Biol Cell Volume: 31 Page(s): 2718 - 2732
    11/15/2020 Authors: Pollitt SL; Myers KR; Yoo J; Zheng JQ
  • The Nebulin Family LIM and SH3 Proteins Regulate Postsynaptic Development and Function.
    J Neurosci Volume: 40 Page(s): 526 - 541
    01/15/2020 Authors: Myers KR; Yu K; Kremerskothen J; Butt E; Zheng JQ
  • Spontaneous Local Calcium Transients Regulate Oligodendrocyte Development in Culture through Store-Operated Ca2+ Entry and Release.
    eNeuro Volume: 7
    01/01/2020 Authors: Rui Y; Pollitt SL; Myers KR; Feng Y; Zheng JQ
  • Regulation of dendritic complexity and synapse formation by the actin-bundling protein LASP2.
    Volume: 29
    12/15/2018 Authors: Myers KR; Yu K; Zheng JQ
  • Tropomodulin Isoform-Specific Regulation of Dendrite Development and Synapse Formation.
    J Neurosci Volume: 38 Page(s): 10271 - 10285
    11/28/2018 Authors: Omotade OF; Rui Y; Lei W; Yu K; Hartzell HC; Fowler VM; Zheng JQ
  • A Novel Drosophila Model to Investigate the Long-Term Cellular and Behavioral Consequences of Repetitive Mild Traumatic Brain Injury
    Volume: 84 Page(s): S234 - S235
    10/01/2018 Authors: Behnke JA; Zheng JQ
  • Actin-based growth cone motility and guidance.
    Mol Cell Neurosci Volume: 84 Page(s): 4 - 10
    10/01/2017 Authors: Omotade OF; Pollitt SL; Zheng JQ
  • The Conserved, Disease-Associated RNA Binding Protein dNab2 Interacts with the Fragile X Protein Ortholog in Drosophila Neurons.
    Cell Rep Volume: 20 Page(s): 1372 - 1384
    08/08/2017 Authors: Bienkowski RS; Banerjee A; Rounds JC; Rha J; Omotade OF; Gross C; Morris KJ; Leung SW; Pak C; Jones SK
  • Phosphoinositide-dependent enrichment of actin monomers in dendritic spines regulates synapse development and plasticity.
    J Cell Biol Volume: 216 Page(s): 2551 - 2564
    08/07/2017 Authors: Lei W; Myers KR; Rui Y; Hladyshau S; Tsygankov D; Zheng JQ
  • Super-Resolution Microscopy Reveals a Nanoscale Organization of Acetylcholine Receptors for Trans-Synaptic Alignment at Neuromuscular Synapses.
    eNeuro Volume: 4
    01/01/2017 Authors: York AL; Zheng JQ
  • Novel functions of LIM and SH3 domain proteins in regulating dendritic development and synapse formation.
    Volume: 28
    01/01/2017 Authors: Myers KR; Zheng JQ
  • p39 Is Responsible for Increasing Cdk5 Activity during Postnatal Neuron Differentiation and Governs Neuronal Network Formation and Epileptic Responses.
    J Neurosci Volume: 36 Page(s): 11283 - 11294
    11/02/2016 Authors: Li W; Allen ME; Rui Y; Ku L; Liu G; Bankston AN; Zheng JQ; Feng Y
  • Oligodendroglial defects during quakingviable cerebellar development.
    Dev Neurobiol Volume: 76 Page(s): 972 - 982
    09/01/2016 Authors: Myers KR; Liu G; Feng Y; Zheng JQ
  • Amyloid oligomers elicit mitochondrial transport defects and fragmentation in a time-dependent and pathway-specific manner.
    Mol Brain Volume: 9 Page(s): 79
    08/17/2016 Authors: Rui Y; Zheng JQ
  • Actin cytoskeleton in dendritic spine development and plasticity.
    Curr Opin Neurobiol Volume: 39 Page(s): 86 - 92
    08/01/2016 Authors: Lei W; Omotade OF; Myers KR; Zheng JQ
  • Two functionally distinct sources of actin monomers supply the leading edge of lamellipodia.
    Cell Rep Volume: 11 Page(s): 433 - 445
    04/21/2015 Authors: Vitriol EA; McMillen LM; Kapustina M; Gomez SM; Vavylonis D; Zheng JQ
  • A critical role for STIM1 in filopodial calcium entry and axon guidance.
    Mol Brain Volume: 6 Page(s): 51
    12/01/2013 Authors: Shim S; Zheng JQ; Ming G-L
  • Instantaneous inactivation of cofilin reveals its function of F-actin disassembly in lamellipodia.
    Mol Biol Cell Volume: 24 Page(s): 2238 - 2247
    07/01/2013 Authors: Vitriol EA; Wise AL; Berginski ME; Bamburg JR; Zheng JQ
  • Dynamic localization of G-actin during membrane protrusion in neuronal motility.
    Curr Biol Volume: 23 Page(s): 1046 - 1056
    06/17/2013 Authors: Lee CW; Vitriol EA; Shim S; Wise AL; Velayutham RP; Zheng JQ
  • Activity-dependent regulation of dendritic growth and maintenance by glycogen synthase kinase 3.
    Nat Commun Volume: 4 Page(s): 2628
    01/01/2013 Authors: Rui Y; Myers KR; Yu K; Wise A; De Blas AL; Hartzell HC; Zheng JQ
  • Dynamic Localization of G-Actin during Membrane Protrusion
    MOLECULAR BIOLOGY OF THE CELL Volume: 24
    01/01/2013 Authors: Vitriol EA; Zheng JQ
  • Micro-organization of acetylcholine receptor clusters and its association with the actin cytoskeleton at the neuromuscular junction
    MOLECULAR BIOLOGY OF THE CELL Volume: 24
    01/01/2013 Authors: York A; Zheng JQ
  • Identification Of Small Molecule TRABID Deubiquitinase Inhibitors By Computation-Based Virtual Screen.
    BMC Chem Biol Volume: 12 Page(s): 4
    05/14/2012 Authors: Shi T; Bao J; Wang NX; Zheng J; Wu D
  • Growth cone travel in space and time: the cellular ensemble of cytoskeleton, adhesion, and membrane.
    Neuron Volume: 73 Page(s): 1068 - 1081
    03/22/2012 Authors: Vitriol EA; Zheng JQ
  • Regulation of chemotropic guidance of nerve growth cones by microRNA.
    Mol Brain Volume: 4 Page(s): 40
    11/03/2011 Authors: Han L; Wen Z; Lynn RC; Baudet M-L; Holt CE; Sasaki Y; Bassell GJ; Zheng JQ
  • Actin capping protein is required for dendritic spine development and synapse formation.
    J Neurosci Volume: 31 Page(s): 10228 - 10233
    07/13/2011 Authors: Fan Y; Tang X; Vitriol E; Chen G; Zheng JQ
  • PSD-95 alters microtubule dynamics via an association with EB3.
    J Neurosci Volume: 31 Page(s): 1038 - 1047
    01/19/2011 Authors: Sweet ES; Previtera ML; Fernndez JR; Charych EI; Tseng C-Y; Kwon M; Starovoytov V; Zheng JQ; Firestein BL
  • Chromophore Assisted Laser Inactivation of ADF/Cofilin Demonstrates Its Function of Filament Severing and Depolymerization in Regulating F-actin Networks.
    Volume: 22
    01/01/2011 Authors: Vitriol EA; Zheng JQ
  • Actin capping protein in dendritic spine development and synapse formation
    NEUROSCIENCE RESEARCH Volume: 71 Page(s): E60 - E60
    01/01/2011 Authors: Fan Y; Tang X; Vitriol E; Chen G; Zheng J
  • Dynamic Localization of Actin Monomers Drives Actin-based Membrane Protrusion and Directional Steering of Nerve Growth Cones.
    Volume: 22
    01/01/2011 Authors: Lee C; Zheng J
  • ADF/cofilin-mediated actin dynamics regulate AMPA receptor trafficking during synaptic plasticity.
    Nat Neurosci Volume: 13 Page(s): 1208 - 1215
    10/01/2010 Authors: Gu J; Lee CW; Fan Y; Komlos D; Tang X; Sun C; Yu K; Hartzell HC; Chen G; Bamburg JR
  • Distinct 3'UTRs differentially regulate activity-dependent translation of brain-derived neurotrophic factor (BDNF).
    Proc Natl Acad Sci U S A Volume: 107 Page(s): 15945 - 15950
    09/07/2010 Authors: Lau AG; Irier HA; Gu J; Tian D; Ku L; Liu G; Xia M; Fritsch B; Zheng JQ; Dingledine R
  • Phosphorylation of zipcode binding protein 1 is required for brain-derived neurotrophic factor signaling of local beta-actin synthesis and growth cone turning.
    J Neurosci Volume: 30 Page(s): 9349 - 9358
    07/14/2010 Authors: Sasaki Y; Welshhans K; Wen Z; Yao J; Xu M; Goshima Y; Zheng JQ; Bassell GJ
  • Inhibition of AMPA receptor trafficking at hippocampal synapses by beta-amyloid oligomers: the mitochondrial contribution.
    Mol Brain Volume: 3 Page(s): 10
    03/26/2010 Authors: Rui Y; Gu J; Yu K; Hartzell HC; Zheng JQ
  • Microtubules in Dendritic Spine Development and Plasticity.
    Open Neurosci J Volume: 3 Page(s): 128 - 133
    12/25/2009 Authors: Gu J; Zheng JQ
  • Regulation of acetylcholine receptor clustering by ADF/cofilin-directed vesicular trafficking.
    Nat Neurosci Volume: 12 Page(s): 848 - 856
    07/01/2009 Authors: Lee CW; Han J; Bamburg JR; Han L; Lynn R; Zheng JQ
  • Microtubules in dendritic spine development.
    J Neurosci Volume: 28 Page(s): 12120 - 12124
    11/12/2008 Authors: Gu J; Firestein BL; Zheng JQ
  • BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin.
    J Cell Biol Volume: 178 Page(s): 107 - 119
    07/02/2007 Authors: Wen Z; Han L; Bamburg JR; Shim S; Ming G-L; Zheng JQ
  • Surfing on calcium waves.
    Neuron Volume: 54 Page(s): 502 - 505
    05/24/2007 Authors: Alder J; Zheng JQ
  • Spatial targeting of type II protein kinase A to filopodia mediates the regulation of growth cone guidance by cAMP.
    J Cell Biol Volume: 176 Page(s): 101 - 111
    01/01/2007 Authors: Han J; Han L; Tiwari P; Wen Z; Zheng JQ
  • Calcium signaling in neuronal motility.
    Annu Rev Cell Dev Biol Volume: 23 Page(s): 375 - 404
    01/01/2007 Authors: Zheng JQ; Poo M-M
  • Membrane lipid rafts and their role in axon guidance.
    Adv Exp Med Biol Volume: 621 Page(s): 144 - 155
    01/01/2007 Authors: Guirland C; Zheng JQ
  • Activity-independent regulation of dendrite patterning by postsynaptic density protein PSD-95.
    J Neurosci Volume: 26 Page(s): 10164 - 10176
    10/04/2006 Authors: Charych EI; Akum BF; Goldberg JS; Jrnsten RJ; Rongo C; Zheng JQ; Firestein BL
  • An essential role for beta-actin mRNA localization and translation in Ca2+-dependent growth cone guidance.
    Nat Neurosci Volume: 9 Page(s): 1265 - 1273
    10/01/2006 Authors: Yao J; Sasaki Y; Wen Z; Bassell GJ; Zheng JQ
  • The molecular basis for calcium-dependent axon pathfinding.
    Nat Rev Neurosci Volume: 7 Page(s): 115 - 125
    02/01/2006 Authors: Gomez TM; Zheng JQ
  • Lipid rafts mediate chemotropic guidance of nerve growth cones.
    Neuron Volume: 42 Page(s): 51 - 62
    04/08/2004 Authors: Guirland C; Suzuki S; Kojima M; Lu B; Zheng JQ
  • Frequency modulation of synchronized Ca2+ spikes in cultured hippocampal networks through G-protein-coupled receptors.
    J Neurosci Volume: 23 Page(s): 4156 - 4163
    05/15/2003 Authors: Liu Z; Geng L; Li R; He X; Zheng JQ; Xie Z
  • Direct cAMP signaling through G-protein-coupled receptors mediates growth cone attraction induced by pituitary adenylate cyclase-activating polypeptide.
    J Neurosci Volume: 23 Page(s): 2274 - 2283
    03/15/2003 Authors: Guirland C; Buck KB; Gibney JA; DiCicco-Bloom E; Zheng JQ
  • Cytoskeletal dynamics underlying collateral membrane protrusions induced by neurotrophins in cultured Xenopus embryonic neurons.
    J Neurobiol Volume: 54 Page(s): 393 - 405
    02/05/2003 Authors: Gibney J; Zheng JQ
  • Growth cone turning induced by direct local modification of microtubule dynamics.
    J Neurosci Volume: 22 Page(s): 9358 - 9367
    11/01/2002 Authors: Buck KB; Zheng JQ
  • Autocrine expression and ontogenetic functions of the PACAP ligand/receptor system during sympathetic development.
    Dev Biol Volume: 219 Page(s): 197 - 213
    03/15/2000 Authors: DiCicco-Bloom E; Deutsch PJ; Maltzman J; Zhang J; Pintar JE; Zheng J; Friedman WF; Zhou X; Zaremba T
  • Turning of nerve growth cones induced by localized increases in intracellular calcium ions.
    Nature Volume: 403 Page(s): 89 - 93
    01/06/2000 Authors: Zheng JQ
  • cAMP-mediated regulation of neurotrophin-induced collapse of nerve growth cones.
    J Neurosci Volume: 18 Page(s): 4973 - 4984
    07/01/1998 Authors: Wang Q; Zheng JQ
  • Acute morphogenic and chemotropic effects of neurotrophins on cultured embryonic Xenopus spinal neurons.
    J Neurosci Volume: 17 Page(s): 7860 - 7871
    10/15/1997 Authors: Ming GL; Lohof AM; Zheng JQ
  • Effects of cytochalasin treatment on short-term synaptic plasticity at developing neuromuscular junctions in frogs.
    J Physiol Volume: 491 ( Pt 1) Page(s): 187 - 195
    02/15/1996 Authors: Wang XH; Zheng JQ; Poo MM
  • Essential role of filopodia in chemotropic turning of nerve growth cone induced by a glutamate gradient.
    J Neurosci Volume: 16 Page(s): 1140 - 1149
    02/01/1996 Authors: Zheng JQ; Wan JJ; Poo MM
  • Calcium and chemotropic turning of nerve growth cones.
    Perspect Dev Neurobiol Volume: 4 Page(s): 205 - 213
    01/01/1996 Authors: Zheng JQ; Poo MM; Connor JA
  • Spontaneous quantal transmitter secretion from myocytes and fibroblasts: comparison with neuronal secretion.
    J Neurosci Volume: 15 Page(s): 2826 - 2838
    04/01/1995 Authors: Girod R; Popov S; Alder J; Zheng JQ; Lohof A; Poo MM
  • Long-range signaling in growing neurons after local elevation of cyclic AMP-dependent activity.
    J Cell Biol Volume: 127 Page(s): 1693 - 1701
    12/01/1994 Authors: Zheng JQ; Zheng Z; Poo M
  • Turning of nerve growth cones induced by neurotransmitters.
    Nature Volume: 368 Page(s): 140 - 144
    03/10/1994 Authors: Zheng JQ; Felder M; Connor JA; Poo MM
  • CHEMOTACTIC RESPONSE OF GROWTH CONES IN A GRADIENT OF ACETYLCHOLINE
    MOLECULAR BIOLOGY OF THE CELL Volume: 3 Page(s): A193 - A193
    09/01/1992 Authors: ZHENG JQ; FELDER M; ZHENG ZW; LOHOF A; POO MM
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