Debayan Dey
- Department of Biochemistry
Assistant Professor
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Emory University School of Medicine
4029 RRC, Department of Biochemistry
1510 Clifton Road
Overview
Our research focuses on mechanistic studies of bacterial antibiotic resistance machineries to understand their structure and dynamics, with the aim of developing targeted inhibitors. We investigate two macromolecular complexes: Bacterial RND efflux pumps and Ribosomal RNA methyltransferases. Additionally, we study allosteric communication, mechanism of gain and loss of function, and other structural dynamics in various clinically important proteins (Human OAS1, OASL, CARD11, Trm10) using computational methods.
We work on developing first-of-class inhibitors against efflux pumps and RNA methyltransferases targeting clinically important antibiotic-resistant pathogens. Our approach combines biophysical, biochemical, and structural techniques such as cryo-EM and X-ray crystallography. Our computational methods include phylogenetics, computer-aided drug discovery, molecular dynamics simulations, protein engineering, and machine learning. By integrating experimental and computational approaches, we aim to uncover complex mechanistic insights.
Academic Appointment
- Assistant Professor, Department of Biochemistry, Emory University School of Medicine
Education
Degrees
- Ph.D. in Structural Biology from Indian Institute of Science
- M.S. in Biophysics and Bioinformatics from University of Calcutta
- B.S. in Physics (Honors) from Presidency College, University of Calcutta
Research
Publications
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DISTANT RIBOSE 2'-O-METHYLATION OF 23S rRNA HELIX 69 PRE-ORDERS THE CAPREOMYCIN DRUG BINDING POCKET AT THE RIBOSOME SUBUNIT INTERFACE.
11/05/2024 Authors: Nandi S; Dey D; Srinivas P; Dunham CM; Conn GL -
Genomic characterization of antibiotic-resistant Staphylococcus epidermidis with observed shifts in optimal temperature.
J Appl Microbiol Volume: 135
10/03/2024 Authors: Boyd SM; Chacon-Barahona JA; Mira P; Dey D; Chun D; Xue C; Pulido S; Yeh P -
Structural and functional diversity of Resistance-Nodulation-Division (RND) efflux pump transporters with implications for antimicrobial resistance.
Microbiol Mol Biol Rev Volume: 88 Page(s): e0008923
09/26/2024 Authors: Kavanaugh LG; Dey D; Shafer WM; Conn GL -
Cumulative phylogenetic, sequence and structural analysis of Insulin superfamily proteins provide unique structure-function insights.
Mol Inform Volume: 43 Page(s): e202300160
09/01/2024 Authors: Rao SS; Kundapura SV; Dey D; Palaniappan C; Sekar K; Kulal A; Ramagopal UA -
Di-berberine conjugates as chemical probes of Pseudomonas aeruginosa MexXY-OprM efflux function and inhibition.
bioRxiv
09/19/2023 Authors: Kavanaugh LG; Mahoney AR; Dey D; Wuest WM; Conn GL -
30S subunit recognition and G1405 modification by the aminoglycoside-resistance 16S ribosomal RNA methyltransferase RmtC.
Proc Natl Acad Sci U S A Volume: 120 Page(s): e2304128120
06/20/2023 Authors: Srinivas P; Nosrati M; Zelinskaya N; Dey D; Comstock LR; Dunham CM; Conn GL -
30S subunit recognition and G1405 modification by the aminoglycoside-resistance 16S ribosomal RNA methyltransferase RmtC.
bioRxiv
04/30/2023 Authors: Srinivas P; Nosrati M; Zelinskaya N; Dey D; Comstock LR; Dunham CM; Conn GL -
Ribosome-targeting antibiotics and resistance via ribosomal RNA methylation.
RSC Med Chem Volume: 14 Page(s): 624 - 643
04/26/2023 Authors: Jeremia L; Deprez BE; Dey D; Conn GL; Wuest WM -
Tied up in knots: Untangling substrate recognition by the SPOUT methyltransferases.
J Biol Chem Volume: 298 Page(s): 102393
10/01/2022 Authors: Strassler SE; Bowles IE; Dey D; Jackman JE; Conn GL -
50S subunit recognition and modification by the Mycobacterium tuberculosis ribosomal RNA methyltransferase TlyA.
Proc Natl Acad Sci U S A Volume: 119 Page(s): e2120352119
04/05/2022 Authors: Laughlin ZT; Nandi S; Dey D; Zelinskaya N; Witek MA; Srinivas P; Nguyen HA; Kuiper EG; Comstock LR; Dunham CM