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Profile

Charles Bou Nader

  • Department of Biochemistry
    Assistant Professor
  • (404) 712-1756
  • charles.bou.nader@emory.edu
  • The Bou-Nader lab
  • See full list of publications here
  • Emory University School of Medicine
    Biochemistry
    1510 Clifton Road NE room 4027
Head shot of Charles Bou Nader

Overview

We focus on mechanistic studies of R-loops and ribonucleoprotein complexes to understand how these assemblies control gene expression and genomic integrity in human health and diseases.

To tackle this important challenge, we use cutting edge biochemical, biophysical, and structural methods such as cryogenic electron microscopy (cryo-EM), X-ray crystallography, and atomic force microscopy (AFM) to define how RNA and RNPs deregulation causes diseases such as neurological disorders, cancers, and autoimmune disorders and their roles in the virus-host interface.

Academic Appointment

  • Assistant Professor, Biochemistry, Emory University
  • Postdoctoral Fellow, National Institutes of Health

Education

Degrees

  • PhD from Sorbonne University
  • MS from Sorbonne University
  • MS from University of Lille
  • BS from Saint Joseph University

Research

Publications

  • Structures of complete HIV-1 TAR RNA portray a dynamic platform poised for protein binding and structural remodeling.
    Nat Commun Volume: 16 Page(s): 2252
    03/06/2025 Authors: Bou-Nader C; Link KA; Suddala KC; Knutson JR; Zhang J
  • Structural basis of MALAT1 RNA maturation and mascRNA biogenesis.
    Nat Struct Mol Biol Volume: 31 Page(s): 1655 - 1668
    11/01/2024 Authors: Skeparnias I; Bou-Nader C; Anastasakis DG; Fan L; Wang Y-X; Hafner M; Zhang J
  • Gcn2 structurally mimics and functionally repurposes the HisRS enzyme for the integrated stress response.
    Proc Natl Acad Sci U S A Volume: 121 Page(s): e2409628121
    08/27/2024 Authors: Bou-Nader C; Gaikwad S; Bahmanjah S; Zhang F; Hinnebusch AG; Zhang J
  • Integrative Approach to Probe Alternative Redox Mechanisms in RNA Modifications.
    Acc Chem Res Volume: 56 Page(s): 3142 - 3152
    11/21/2023 Authors: Bou-Nader C; Pecqueur L; de Crcy-Lagard V; Hamdane D
  • S9.6 Antibody-Enzyme Conjugates for the Detection of DNA-RNA Hybrids.
    Bioconjug Chem Volume: 34 Page(s): 834 - 844
    05/17/2023 Authors: Wei E; Bou-Nader C; Perry ML; Fattah R; Zhang J; Leppla SH; Bothra A
  • Shigella IpaA mediates actin bundling through diffusible vinculin oligomers with activation imprint.
    Cell Rep Volume: 42 Page(s): 112405
    04/25/2023 Authors: Valencia-Gallardo C; Aguilar-Salvador D-I; Khakzad H; Cocom-Chan B; Bou-Nader C; Velours C; Zarrouk Y; Le Clainche C; Malosse C; Lima DB
  • Structural basis of R-loop recognition by the S9.6 monoclonal antibody.
    Nat Commun Volume: 13 Page(s): 1641
    03/28/2022 Authors: Bou-Nader C; Bothra A; Garboczi DN; Leppla SH; Zhang J
  • Rational engineering enables co-crystallization and structural determination of the HIV-1 matrix-tRNA complex.
    STAR Protoc Volume: 3 Page(s): 101056
    03/18/2022 Authors: Bou-Nader C; Zhang J
  • HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization.
    Cell Host Microbe Volume: 29 Page(s): 1421 - 1436.e7
    09/08/2021 Authors: Bou-Nader C; Muecksch F; Brown JB; Gordon JM; York A; Peng C; Ghirlando R; Summers MF; Bieniasz PD; Zhang J
  • An enzymatic activation of formaldehyde for nucleotide methylation.
    Nat Commun Volume: 12 Page(s): 4542
    07/27/2021 Authors: Bou-Nader C; Stull FW; Pecqueur L; Simon P; Gurineau V; Royant A; Fontecave M; Lombard M; Palfey BA; Hamdane D
  • Structural Insights into RNA Dimerization: Motifs, Interfaces and Functions.
    Molecules Volume: 25
    06/23/2020 Authors: Bou-Nader C; Zhang J
  • Crystal structure of an adenovirus virus-associated RNA.
    Nat Commun Volume: 10 Page(s): 2871
    06/28/2019 Authors: Hood IV; Gordon JM; Bou-Nader C; Henderson FE; Bahmanjah S; Zhang J
  • Conformational Stability Adaptation of a Double-Stranded RNA-Binding Domain to Transfer RNA Ligand.
    Biochemistry Volume: 58 Page(s): 2463 - 2473
    05/21/2019 Authors: Bou-Nader C; Pecqueur L; Barraud P; Fontecave M; Tisn C; Sacquin-Mora S; Hamdane D
  • The search for a PKR code-differential regulation of protein kinase R activity by diverse RNA and protein regulators.
    RNA Volume: 25 Page(s): 539 - 556
    05/01/2019 Authors: Bou-Nader C; Gordon JM; Henderson FE; Zhang J
  • Ultrafast photoinduced flavin dynamics in the unusual active site of the tRNA methyltransferase TrmFO.
    Phys Chem Chem Phys Volume: 21 Page(s): 8743 - 8756
    04/24/2019 Authors: Dozova N; Lacombat F; Bou-Nader C; Hamdane D; Plaza P
  • Molecular basis for transfer RNA recognition by the double-stranded RNA-binding domain of human dihydrouridine synthase 2.
    Nucleic Acids Res Volume: 47 Page(s): 3117 - 3126
    04/08/2019 Authors: Bou-Nader C; Barraud P; Pecqueur L; Prez J; Velours C; Shepard W; Fontecave M; Tisn C; Hamdane D
  • Shigella IpaA Binding to Talin Stimulates Filopodial Capture and Cell Adhesion.
    Cell Rep Volume: 26 Page(s): 921 - 932.e6
    01/22/2019 Authors: Valencia-Gallardo C; Bou-Nader C; Aguilar-Salvador D-I; Carayol N; Quenech'Du N; Pecqueur L; Park H; Fontecave M; Izard T; Tran Van Nhieu G
  • Dissociation of the Dimer of the Intrinsically Disordered Domain of RNase Y upon Antibody Binding.
    Biophys J Volume: 115 Page(s): 2102 - 2113
    12/04/2018 Authors: Hardouin P; Velours C; Bou-Nader C; Assrir N; Laalami S; Putzer H; Durand D; Golinelli-Pimpaneau B
  • Electrostatic Potential in the tRNA Binding Evolution of Dihydrouridine Synthases.
    Biochemistry Volume: 57 Page(s): 5407 - 5414
    09/18/2018 Authors: Bou-Nader C; Brgeon D; Pecqueur L; Fontecave M; Hamdane D
  • Unveiling structural and functional divergences of bacterial tRNA dihydrouridine synthases: perspectives on the evolution scenario.
    Nucleic Acids Res Volume: 46 Page(s): 1386 - 1394
    02/16/2018 Authors: Bou-Nader C; Montmont H; Gurineau V; Jean-Jean O; Brgeon D; Hamdane D
  • Power of protein/tRNA functional assembly against aberrant aggregation.
    Phys Chem Chem Phys Volume: 19 Page(s): 28014 - 28027
    10/25/2017 Authors: Bou-Nader C; Pecqueur L; Cornu D; Lombard M; Dezi M; Nicaise M; Velours C; Fontecave M; Hamdane D
  • Enzyme Activation with a Synthetic Catalytic Co-enzyme Intermediate: Nucleotide Methylation by Flavoenzymes.
    Angew Chem Int Ed Engl Volume: 56 Page(s): 12523 - 12527
    10/02/2017 Authors: Bou-Nader C; Cornu D; Guerineau V; Fogeron T; Fontecave M; Hamdane D
  • An extended dsRBD is required for post-transcriptional modification in human tRNAs.
    Nucleic Acids Res Volume: 43 Page(s): 9446 - 9456
    10/30/2015 Authors: Bou-Nader C; Pecqueur L; Bregeon D; Kamah A; Gurineau V; Golinelli-Pimpaneau B; Guimares BG; Fontecave M; Hamdane D
  • Flavin-Protein Complexes: Aromatic Stacking Assisted by a Hydrogen Bond.
    Biochemistry Volume: 54 Page(s): 4354 - 4364
    07/21/2015 Authors: Hamdane D; Bou-Nader C; Cornu D; Hui-Bon-Hoa G; Fontecave M
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